Coding

Part:BBa_K4880002:Design

Designed by: Xinran Xia   Group: iGEM23_BASIS-China   (2023-08-25)


AgBS


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1681
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1681
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1276
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1681
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1681
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 64
    Illegal BsaI.rc site found at 505
    Illegal BsaI.rc site found at 1288


Design Notes

-


Source

This sequence is derived from the Abies grandis genome, and it is adapted to the cyanobacteria codon.


References

Blanc-Garin, V., Chenebault, C., Diaz-Santos, E., Vincent, M., Sassi, J.-F., Cassier-Chauvat, C., & Chauvat, F. (2022). Exploring the potential of the model cyanobacterium Synechocystis PCC 6803 for the photosynthetic production of various high-value terpenes. Biotechnology for Biofuels and Bioproducts, 15(1). https://doi.org/10.1186/s13068-022-02211-0